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Anti-ICAM-1/CD54 Antibody (3B313)
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产品编号 TMAY-03695
别名 P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2
Anti-ICAM-1/CD54 Antibody (3B313) 是一种 Rabbit 抗体，靶向 ICAM-1/CD54。Anti-ICAM-1/CD54 Antibody (3B313) 可用于 ELISA(Cap),FCM,ICC/IF。
Anti-ICAM-1/CD54 Antibody (3B313)
Anti-ICAM-1/CD54 Antibody (3B313)
还可以产品编号 TMAY-03695 别名 P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2
Anti-ICAM-1/CD54 Antibody (3B313) 是一种 Rabbit 抗体，靶向 ICAM-1/CD54。Anti-ICAM-1/CD54 Antibody (3B313) 可用于 ELISA(Cap),FCM,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μL | ¥ 780 | 5日内发货 | |
| 100 μL | ¥ 2,284 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-ICAM-1/CD54 Antibody (3B313) is a Rabbit antibody targeting ICAM-1/CD54. Anti-ICAM-1/CD54 Antibody (3B313) can be used in ELISA(Cap),FCM,ICC/IF. |
| 别名 | P3.58, intercellular adhesion molecule 1, ICAM-1, CD54, BB2 |
| Ig Type | Rabbit IgG |
| 克隆号 | 3B313 |
| 交叉反应 | Rat |
| 验证活性 | 1. Flow cytometric analysis of Rat ICAM1 expression on SD rat splenocytes. Cells were stained with purified anti-Rat ICAM1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of rat ICAM1 in rat splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-rat ICAM1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). |
| 应用 | ELISA(Cap),FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Rat ICAM-1 / CD54 (rR ICAM-1 / CD54; TMPY-02017; Q00238-1; Met1-Thr493). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/FITC/PE |
| 可提供抗体种类 | 4 |
| 免疫原 | Recombinant Protein: Rat ICAM-1 / CD54 protein (TMPY-02017) |
| 抗原种属 | Rat |
| 研究领域 | Neuroinflammation |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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