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Anti-Iba1 Antibody (1X229)
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产品编号 TMAC-02080
别名 IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1
Anti-Iba1 Antibody (1X229) 是一种 Rabbit 抗体，靶向 Iba1。Anti-Iba1 Antibody (1X229) 可用于 WB,ICC,IF,IHC,FCM,IP。
Anti-Iba1 Antibody (1X229)
Anti-Iba1 Antibody (1X229)
还可以产品编号 TMAC-02080 别名 IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1
Anti-Iba1 Antibody (1X229) 是一种 Rabbit 抗体，靶向 Iba1。Anti-Iba1 Antibody (1X229) 可用于 WB,ICC,IF,IHC,FCM,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-Iba1 Antibody (1X229) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (1X229) can be used in WB,ICC,IF,IHC,FCM,IP. |
| 别名 | IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1 |
| Ig Type | IgG |
| 克隆号 | 1X229 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Iba1 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Iba1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Iba1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Iba1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Iba1 antibody. Counter stained with hematoxylin. 6. ICC staining Iba1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of THP-1 cells with Iba1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). 8. All lanes : Iba1 Antibody at 1/2k dilution, Lane 1: Mouse liver lysates, Lane 2: Mouse spleen lysates, Lane 3: Mouse lung lysates, Lane 4: Rat liver lysates, Lane 5: Rat spleen lysates, Lane 6: Rat lung lysates Lysates/proteins at 20 μg per lane. Secondary All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution, Predicted band size: 17 kDa, Observed band size: 17 kDa, Exposure time: 120 seconds. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:100-500; ICC:IF: 1:50-100; IP: 1:10-50; FCM: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC). |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 17 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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