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Anti-Human CD63 Antibody (1Y320) 是一种 Mouse 抗体,靶向 Human CD63。Anti-Human CD63 Antibody (1Y320) 可用于 FCM,ICC/IF。
Anti-Human CD63 Antibody (1Y320) 是一种 Mouse 抗体,靶向 Human CD63。Anti-Human CD63 Antibody (1Y320) 可用于 FCM,ICC/IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μg | ¥ 1,865 | 5日内发货 |
| 产品描述 | Anti-Human CD63 Antibody (1Y320) is a Mouse antibody targeting Human CD63. Anti-Human CD63 Antibody (1Y320) can be used in FCM,ICC/IF. |
| Ig Type | Mouse IgG1, k |
| 克隆号 | 1Y320 |
| 反应种属 | Human |
| 验证活性 | 1. 4% Paraformaldehyde-fixed A375 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (human CD63) monoclonal Antibody, unconjugated (TMAB-07416) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control. 2. The A375 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-Human CD63 antibody (TMAB-07416): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| 应用 | FCMICC/IF |
| 推荐剂量 | FCM: 1μg/Test; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. |
| 组织特异性 | Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein G purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4). |
| 研究背景 | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012] |
| 基因名称 | CD63 |
| 基因ID | |
| 蛋白名称 | CD63 antigen |
| 功能 | This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. |
| 分子量 | Theoretical: 26 kDa. Actual: 30-50 kDa. |
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |