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Anti-Human CD63 Antibody (1Y320)

货号 TMAB-07416 一键复制产品信息
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Anti-Human CD63 Antibody (1Y320) 是一种 Mouse 抗体,靶向 Human CD63。Anti-Human CD63 Antibody (1Y320) 可用于 FCM,ICC/IF。

Anti-Human CD63 Antibody (1Y320)

一键复制产品信息
Rating icon 还可以
货号 TMAB-07416

Anti-Human CD63 Antibody (1Y320) 是一种 Mouse 抗体,靶向 Human CD63。Anti-Human CD63 Antibody (1Y320) 可用于 FCM,ICC/IF。

Anti-Human CD63 Antibody (1Y320)
规格价格库存数量
100 μg
¥ 1,865
5日内发货
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产品介绍


生物活性
产品描述
Anti-Human CD63 Antibody (1Y320) is a Mouse antibody targeting Human CD63. Anti-Human CD63 Antibody (1Y320) can be used in FCM,ICC/IF.
Ig Type
Mouse IgG1, k
克隆号
1Y320
反应种属
Human
验证活性
1. 4% Paraformaldehyde-fixed A375 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (human CD63) monoclonal Antibody, unconjugated (TMAB-07416) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The A375 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-Human CD63 antibody (TMAB-07416): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
应用FCMICC/IF
推荐剂量
FCM: 1μg/Test; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Mouse
亚细胞定位Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
组织特异性Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
构建方式Hybridoma Monoclonal Antibody
纯化方式Protein G purified
性状Liquid
缓冲液0.01M TBS (pH7.4).
研究背景The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
抗原信息
基因名称
CD63
基因ID
蛋白名称
CD63 antigen
功能
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
化学信息
分子量Theoretical: 26 kDa. Actual: 30-50 kDa.
储存&溶解度
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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