Anti-HPV16 E7 Polyclonal Antibody 2 is a Rabbit antibody targeting HPV16 E7. Anti-HPV16 E7 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human (predicted:HPV16)

1. Sample:
hela (Human) Cell Lysate at 30 μg
mcf-7 (Human) Cell Lysate at 30 μg
mda-mb-231 (Human) Cell Lysate at 30 μg
siha (Human) Cell Lysate at 30 μg
recombinant HPV16-E7 protein 100 ng
recombinant HPV16-E7 protein 50 ng
Primary: Anti-HPV16 E7 (TMAB-00886) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kD
Observed band size: 17-20 kD
HPV16-E7 band size: 45 kDa
2. Sample:
Lane 1: HPV16 E7 fusion protein 0.1 μg Lysates
Lane 2: HPV16 E7 fusion protein 0.05 μg Lysates
Primary: Anti-HPV16 E7 (TMAB-00886) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 50 kDa
3. Blank control (black line): Hela.
Primary Antibody (green line): Rabbit Anti-HPV16 E7 antibody (TMAB-00886)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: HPV16 E7 protein