Anti-HP1 gamma Antibody (7G984) is a Mouse antibody targeting HP1 gamma. Anti-HP1 gamma Antibody (7G984) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

7G984

Human,Rat (predicted:Mouse)

1. Sample:
Molt-4 cell (human) Lysate at 30 μg
jurkat cell (human) Lysate at 30 μg
HepG2 cell (human) Lysate at 30 μg
Primary: Anti-HP1 gammA (TMAB-07268) at 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
2. Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HP1 gamma) Monoclonal Antibody, Unconjugated (cell supernatant of TMAB-07268) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (HP1 gamma) Monoclonal Antibody, Unconjugated (cell supernatant of TMAB-07268) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

Recombinant Protein: human HP1 gamma Protein

Human

CBX3

Chromobox protein homolog 3

Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.