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Anti-HNRNPK Antibody (5Q710) 是一种 Rabbit 抗体,靶向 HNRNPK。Anti-HNRNPK Antibody (5Q710) 可用于 WB,ICC/IF,IHC,IP。
Anti-HNRNPK Antibody (5Q710) 是一种 Rabbit 抗体,靶向 HNRNPK。Anti-HNRNPK Antibody (5Q710) 可用于 WB,ICC/IF,IHC,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-HNRNPK Antibody (5Q710) is a Rabbit antibody targeting HNRNPK. Anti-HNRNPK Antibody (5Q710) can be used in WB,ICC/IF,IHC,IP. |
别名 | TUNP, Transformation upregulated nuclear protein, Transformation up regulated nuclear protein, HNRNPK, hnRNP K, Heterogeneous nuclear ribonucleoprotein K, FLJ41122, dC stretch binding protein, CSBP |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of hnRNP K on different lysates using anti-hnRNP K antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: Hela, Lane 3: NIH/3T3. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-hnRNP K antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-hnRNP K antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-hnRNP K antibody. Counter stained with hematoxylin. 5. ICC staining hnRNP K in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining hnRNP K in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining hnRNP K in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 60 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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