Anti-hnRNP U Polyclonal Antibody is a Rabbit antibody targeting hnRNP U. Anti-hnRNP U Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse (predicted:Rat,Pig,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (hnRNP U) Polyclonal Antibody, Unconjugated (TMAB-07226) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-hnRNP U (TMAB-07226) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 90 kD
3. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Human K562 cell lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human 293T cell lysates
Lane 7: Human HepG2 cell lysates
Primary: Anti-hnRNP U (TMAB-07226) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 120 kDa
4. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (hnRNP U) Polyclonal Antibody, Unconjugated (TMAB-07226) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (hnRNP U) polyclonal Antibody, Unconjugated (TMAB-07226) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:50-200

Polyclonal

KLH conjugated synthetic peptide: human hnRNP U

Human

HNRNPU

Heterogeneous nuclear ribonucleoprotein U

Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.