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Anti-Histone H2B type 1-K Antibody (4Z139)
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产品编号 TMAC-01864
Anti-Histone H2B type 1-K Antibody (4Z139) 是一种 Rabbit 抗体，靶向 Histone H2B type 1-K。Anti-Histone H2B type 1-K Antibody (4Z139) 可用于 WB,ICC/IF,IHC,IP,FCM。
Anti-Histone H2B type 1-K Antibody (4Z139)
Anti-Histone H2B type 1-K Antibody (4Z139)
还可以产品编号 TMAC-01864
Anti-Histone H2B type 1-K Antibody (4Z139) 是一种 Rabbit 抗体，靶向 Histone H2B type 1-K。Anti-Histone H2B type 1-K Antibody (4Z139) 可用于 WB,ICC/IF,IHC,IP,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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| 产品描述 | Anti-Histone H2B type 1-K Antibody (4Z139) is a Rabbit antibody targeting Histone H2B type 1-K. Anti-Histone H2B type 1-K Antibody (4Z139) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of Histone H2B on different lysates using anti-Histone H2B antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: NIH/3T3, Lane 3: PC12. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B antibody. Counter stained with hematoxylin. 8. ICC staining Histone H2B in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with Histone H2B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 14 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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