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顶部生物活性储存和溶解信息计算器

Anti-HIF1AN Antibody (9B616)

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Anti-HIF1AN Antibody (9B616)
产品编号 TMAC-01820

Anti-HIF1AN Antibody (9B616) 是一种 Rabbit 抗体，靶向 HIF1AN。Anti-HIF1AN Antibody (9B616) 可用于 WB,ICC,IHC,FCM。

Anti-HIF1AN Antibody (9B616)

Anti-HIF1AN Antibody (9B616)

Rating icon 还可以
Anti-HIF1AN Antibody (9B616)
产品编号 TMAC-01820

Anti-HIF1AN Antibody (9B616) 是一种 Rabbit 抗体，靶向 HIF1AN。Anti-HIF1AN Antibody (9B616) 可用于 WB,ICC,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,485
5日内发货
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产品介绍

生物活性
产品描述
Anti-HIF1AN Antibody (9B616) is a Rabbit antibody targeting HIF1AN. Anti-HIF1AN Antibody (9B616) can be used in WB,ICC,IHC,FCM.
Ig Type
IgG
交叉反应
Human
验证活性
1. Western blot analysis of HIF1AN on A549 and Jurkat cell lysates using anti-HIF1AN antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-HIF1AN antibody. Counter stained with hematoxylin.
3. ICC staining HIF1AN in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining HIF1AN in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. Flow cytometric analysis of LOVO cells with HIF1AN antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
应用
WB
ICC
IHC
FCM
推荐剂量
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1α. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the β-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1α. This hydroxylation event blocks the association of HIF-1α with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1α. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm. The structure of FIH-1 includes a jellyroll-like β-barrel containing ferrous-binding triad residues. The gene encoding human FIH-1 maps to chromosome 10q24.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: human HIF1AN aa 1-200
抗原种属
human
Uniprot ID
Q9NWT6
化学信息
分子量Theoretical: 40 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

Hypoxia-inducible factor asparagine hydroxylaseHypoxia-inducible factor 1-α inhibitorHypoxia-inducible factor 1-alpha inhibitorHypoxia-inducible factor 1-a inhibitorHIF1ANFIH-1FIH1FIH 1Factor inhibiting HIF-1 (FIH-1)

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