Anti-HDAC8 Antibody (6A120) is a Rabbit antibody targeting HDAC8. Anti-HDAC8 Antibody (6A120) can be used in WB,ICC/IF,IHC,IP,FCM.

IgG

Human

1. Western blot analysis of HDAC8 on different lysates using anti-HDAC8 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: K562.
2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-HDAC8 antibody. Counter stained with hematoxylin.
6. ICC staining HDAC8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining HDAC8 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining HDAC8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of K562 cells with HDAC8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100

Monoclonal

Unconjugated

Recombinant Protein