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Anti-H7N9 Hemagglutinin/HA Antibody (3J670)

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产品编号 TMAY-01613

Anti-H7N9 Hemagglutinin/HA Antibody (3J670) 是一种 Rabbit 抗体,靶向 H7N9 Hemagglutinin/HA。Anti-H7N9 Hemagglutinin/HA Antibody (3J670) 可用于 ELISA,ELISA(Cap),FCM,HemagglutininInhibition(HI)。

Anti-H7N9 Hemagglutinin/HA Antibody (3J670)

Anti-H7N9 Hemagglutinin/HA Antibody (3J670)

Rating icon 还可以
产品编号 TMAY-01613

Anti-H7N9 Hemagglutinin/HA Antibody (3J670) 是一种 Rabbit 抗体,靶向 H7N9 Hemagglutinin/HA。Anti-H7N9 Hemagglutinin/HA Antibody (3J670) 可用于 ELISA,ELISA(Cap),FCM,HemagglutininInhibition(HI)。

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产品介绍

生物活性
产品描述
Anti-H7N9 Hemagglutinin/HA Antibody (3J670) is a Rabbit antibody targeting H7N9 Hemagglutinin/HA. Anti-H7N9 Hemagglutinin/HA Antibody (3J670) can be used in ELISA,ELISA(Cap),FCM,HemagglutininInhibition(HI).
Ig Type
Monoclonal Rabbit IgG
克隆号
3J670
验证活性
Flow cytometric analysis of Purified anti-H7N9-HA antibody on 293 transfected cells. 293 cells were transfected with plasmid DNA of H7N7 (A/Netherlands/219/2003) Hemagglutinin, then the cells were collected at 72 hours posttransfection, and the cell surface expression of HA were measured by flowcytometry. The transfected cells stained with Purified Rabbit anti-H7N9-HA (Bold line hisgram, 1 μg /test), To demonstrate specificity of staining, the binding of Anti-H7N9 Hemagglutinin/HA Antibody was blocked by the preincubation of the purified antibody with molar excess of recombinant Influenza A H7N9 (A/Shanghai/1/2013) Hemagglutinin (Left panel, 5 μg), and Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin (Right panel, 5 μg) for 1 hour (Dashed line hisgram), then stained with a FITC-conjugated second step antibody, grey line histogram represented negative control.
应用
ELISA,ELISA(Cap),FCM,HemagglutininInhibition(HI)
推荐剂量
ELISA: 0.1-0.2 μg/mL; FCM: 0.5-2 μg/Test; ELISA(Cap): 0.5-4 µg/ml
抗体种类
Monoclonal
宿主来源Rabbit
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
偶联与修饰
偶联
Unconjugated
其他可选修饰符
Biotin/FITC
可提供抗体种类
3
抗原信息
免疫原
0
抗原种属
H7N9
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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