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Anti-H2AFX Polyclonal Antibody 是一种 Rabbit 抗体,靶向 H2AFX。Anti-H2AFX Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-H2AFX Polyclonal Antibody 是一种 Rabbit 抗体,靶向 H2AFX。Anti-H2AFX Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,790 | 5日内发货 |
产品描述 | Anti-H2AFX Polyclonal Antibody is a Rabbit antibody targeting H2AFX. Anti-H2AFX Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Tissue/cell: human glioma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-H2AX/Histone H2A.x Polyclonal Antibody, Unconjugated (TMAB-00826) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Histone H2A.x) Polyclonal Antibody, Unconjugated (TMAB-00826) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (H2AX) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (H2AX) Polyclonal Antibody, Unconjugated (TMAB-00826) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (H2AX) Polyclonal Antibody, Unconjugated (TMAB-00826) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Human MCF-7 cell lysates Lane 4: Human 293T cell lysates Lane 5: Human Jurkat cell lysates Lane 6: Human HeLa cell lysates Lane 7: Human K562 cell lysates Lane 8: Human MOLT4 cell lysates Primary: Anti-H2AX (TMAB-00826) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500 |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Nucleus. Chromosome. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008]. |
免疫原 | KLH conjugated synthetic peptide: human H2AX |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | ChIP antibodies,DNA Damage Recognition,Unmodified,Variants |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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