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别名 GSTtag
Anti-GST tag Antibody (4G528) 是一种 Mouse 抗体,靶向 GST tag。Anti-GST tag Antibody (4G528) 可用于 ELISA, IP, WB。
Anti-GST tag Antibody (4G528) 是一种 Mouse 抗体,靶向 GST tag。Anti-GST tag Antibody (4G528) 可用于 ELISA, IP, WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 100 μg | ¥ 2,438 | 5日内发货 |
| 产品描述 | Anti-GST tag Antibody (4G528) is a Mouse antibody targeting GST tag. Anti-GST tag Antibody (4G528) can be used in ELISA, IP, WB. |
| 别名 | GSTtag |
| Ig Type | IgG1,κ |
| 克隆号 | 4G528 |
| 特异性 | GST localized at the C-terminal, N-terminal, and internal region of GST-tagged fusion proteins |
| 应用 | ELISAIPWB |
| 推荐剂量 | ELISA: 0.05-0.2 µg/ml; IP: 1 µg/ml; WB: 0.1-1.0 µg/ml |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 纯化方式 | Protein A affinity column |
| 性状 | Lyophilized. Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL. |
| 缓冲液 | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| 研究背景 | Schistosoma japonicum glutathione S-transferase (GST) is a 26 kDa enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E.coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | HRP |
| 可提供抗体种类 | 2 |
| 免疫原 | Recombinant Protein: GST protein |
| 储存方式 | The lyophilized product store at -20°C or -80°C for 12 months. The reconstituted antibody can be stored at 2°C-8°C for 2-3 weeks. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | -20°C for 1 year |