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Anti-GRASP65 Antibody (9N637) 是一种 Rabbit 抗体,靶向 GRASP65。Anti-GRASP65 Antibody (9N637) 可用于 WB,ICC,IF,IHC,FCM,IP。
Anti-GRASP65 Antibody (9N637) 是一种 Rabbit 抗体,靶向 GRASP65。Anti-GRASP65 Antibody (9N637) 可用于 WB,ICC,IF,IHC,FCM,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-GRASP65 Antibody (9N637) is a Rabbit antibody targeting GRASP65. Anti-GRASP65 Antibody (9N637) can be used in WB,ICC,IF,IHC,FCM,IP. |
别名 | P65, MGC118897, MGC118894, GRASP65, GRASP 65, GORS1_HUMAN, GORASP1, Gorasp 1, GOLPH5, GOLPH 5, Golgi reassembly-stacking protein of 65 kDa, Golgi reassembly-stacking protein 1, Golgi reassembly stacking protein of 65 kDa, Golgi reassembly stacking protein 1 65kDa, Golgi reassembly stacking protein 1, Golgi reassembly and stacking protein, 65-kD, Golgi reassembly and stacking protein 65 kDa, Golgi reassembly and stacking protein 1, Golgi phosphoprotein 5, Golgi peripheral membrane protein p65, FLJ23443 |
Ig Type | IgG |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of GRASP65 on different cell lysates using anti-GRASP65 at 1/500 dilution. Positive control: Line 1: THP-1, Line 2: SKBR3. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GRASP65 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human uterine tissue using anti-GRASP65 antibody. Counter stained with hematoxylin. 4. ICC staining GRASP65 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining GRASP65 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining GRASP65 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of MCF-7 cells with GRASP65 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 46 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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