Anti-GPC3 Antibody (2J984) is a Mouse antibody targeting GPC3. Anti-GPC3 Antibody (2J984) can be used in ELISA, WB, FCM.

IgG2a

2J984

Human

1. Western Blot
-Positive WB detected in: GPC3 antibody at 1:1000
-Lane 1: Jurkat whole cell lysate
-Lane 2: Mouse liver tissue
-Secondary: Goat polyclonal to Mouse IgG at 1/20000 dilution
-Predicted band size: 65-70KDa
-Observed band size: 65-70 KDa
-Exposure time: 5min
2. Overlay Peak curve showing Jurkat cells stained with TMAH-00501 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Monoclonal

Unconjugated

Recombinant Protein: Human Glypican-3 Protein (25-559AA)

Human

Neuroscience