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Anti-GOLPH2/GOLM1 Antibody (3T18) 是一种 Mouse 抗体,靶向 GOLPH2/GOLM1。Anti-GOLPH2/GOLM1 Antibody (3T18) 可用于 IHC-P,ICC/IF。
Anti-GOLPH2/GOLM1 Antibody (3T18) 是一种 Mouse 抗体,靶向 GOLPH2/GOLM1。Anti-GOLPH2/GOLM1 Antibody (3T18) 可用于 IHC-P,ICC/IF。
产品描述 | Anti-GOLPH2/GOLM1 Antibody (3T18) is a Mouse antibody targeting GOLPH2/GOLM1. Anti-GOLPH2/GOLM1 Antibody (3T18) can be used in IHC-P,ICC/IF. |
Ig Type | Monoclonal Mouse IgG2a |
克隆号 | 3T18 |
交叉反应 | Human |
验证活性 | 1. Immunochemical staining of human GP73 in human uterus with mouse monoclonal antibody (8 µg/mL, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human GP73 in human stomach with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). 3. Immunochemical staining of human GP73 in human liver (cirrhosis) with mouse monoclonal antibody (1:100, formalin-fixed paraffin embedded sections). 4. Immunofluorescence staining of GP73 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with mouse anti-human GP73 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to golgi apparatus . |
应用 | IHC-P,ICC/IF |
推荐剂量 | IHC-P: 5-20 μg/mL; ICC-IF: 1:20-1:100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS |
研究背景 | Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2, and GOLM1, is a protein that belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in the colon, prostate, trachea, and stomach. It is expressed at a lower level in testis, muscle, lymphoid tissues, white blood cells, and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at a low level in the normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncytial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes. |
偶联 | Unconjugated |
免疫原 | Recombinant Human GOLPH2 / GOLM1 Protein |
抗原种属 | Human |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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