- 全部删除
- 您的购物车当前为空
Anti-GOLM1 Antibody (1M115) 是一种抗体,靶向 GOLM1。Anti-GOLM1 Antibody (1M115) 可用于 ELISA, WB, FCM。
Anti-GOLM1 Antibody (1M115) 是一种抗体,靶向 GOLM1。Anti-GOLM1 Antibody (1M115) 可用于 ELISA, WB, FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,300 | 5日内发货 | |
100 μL | ¥ 2,185 | 5日内发货 |
产品描述 | Anti-GOLM1 Antibody (1M115) is an antibody targeting GOLM1. Anti-GOLM1 Antibody (1M115) can be used in ELISA, WB, FCM. |
Ig Type | Rabbit IgG |
克隆号 | 1M115 |
交叉反应 | Human |
验证活性 | 1. Western Blot -Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate -All lanes: GOLM1 antibody at 1.3μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 46, 45 KDa -Observed band size: 80 KDa 2. Overlay histogram showing Hela cells stained with TMAH-00499 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. |
应用 | |
推荐剂量 | WB:1:500-1:5000. |
抗体种类 | Monoclonal |
亚细胞定位 | Golgi apparatus, cis-Golgi network membrane; Single-pass type II membrane protein. Note=Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption. |
组织特异性 | Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in n |
构建方式 | Recombinant Antibody |
纯化方式 | Affinity-chromatography |
性状 | Liquid |
缓冲液 | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
研究背景 | Unknown. Cellular response protein to viral infection. |
偶联 | Unconjugated |
免疫原 | A synthetic peptide: Human GOLM1 |
抗原种属 | Human |
基因名称 | GOLM1 |
基因ID | |
Uniprot ID | |
研究领域 | Signal Transduction |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
评论内容