Anti-GJA1 Polyclonal Antibody 2 is a Rabbit antibody targeting GJA1. Anti-GJA1 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Rabbit)

1. Tissue/cell: human cervix carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Connexin 43 Polyclonal Antibody, Unconjugated (TMAB-00773) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Connexin 43 Polyclonal Antibody, Unconjugated (TMAB-00773) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Blank control (blue): HUVEC cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Connexin 43 antibody (TMAB-00773), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
4. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Mouse Stomach tissue lysates
Primary: Anti-Connexin 43 (TMAB-00773) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 48 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Connexin 43

Human

Cell junction molecules,Cardiac arrhythmias,Gap Junctions