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Anti-GIT1 Antibody (6A39) 是一种 Rabbit 抗体,靶向 GIT1。Anti-GIT1 Antibody (6A39) 可用于 WB,IHC,FCM。
Anti-GIT1 Antibody (6A39) 是一种 Rabbit 抗体,靶向 GIT1。Anti-GIT1 Antibody (6A39) 可用于 WB,IHC,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,490 | 5日内发货 | |
100 μL | ¥ 2,485 | 5日内发货 |
产品描述 | Anti-GIT1 Antibody (6A39) is a Rabbit antibody targeting GIT1. Anti-GIT1 Antibody (6A39) can be used in WB,IHC,FCM. |
别名 | GRK-interacting protein 1, GRK interacting protein 1, GIT1_HUMAN, GIT 1, G protein-coupled receptor kinase-interactor 1, G protein coupled receptor kinase interactor 1, G protein coupled receptor kinase interacting ArfGAP 1, Cool-associated and tyrosine-phosphorylated protein 1, Cool associated and tyrosine phosphorylated protein 1, CAT-1, CaT1, CAT 1, ARF GTPase-activating protein GIT1, ARF GTPase activating protein GIT1, ARF GAP GIT1 |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of GIT1 on A549 cell using anti-GIT1 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GIT1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GIT1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-GIT1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GIT1 antibody. Counter stained with hematoxylin. 6. Flow cytometric analysis of A549 cells with GIT1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
应用 | |
推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade. GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking. GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 85 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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