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顶部生物活性储存和溶解信息计算器

Anti-GCNF Antibody (6U757)

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Anti-GCNF Antibody (6U757)
产品编号 TMAC-01638
别名 RTR, Retinoid receptor-related testis-specific receptor, Retinoid receptor related testis specific receptor, Retinoic acid receptor related testis associated receptor, Orphan nuclear receptor NR6A1, Nuclear receptor subfamily 6 group A member 1, NR6A1_HUMAN, nr6a1, NR61, NR 61, hRTR, hGCNF, Germ cell nuclear factor, GCNF1, GCNF 1, GCNF

Anti-GCNF Antibody (6U757) 是一种 Mouse 抗体，靶向 GCNF。Anti-GCNF Antibody (6U757) 可用于 WB,ICC,IHC,FCM。

Anti-GCNF Antibody (6U757)

Anti-GCNF Antibody (6U757)

Rating icon 还可以
Anti-GCNF Antibody (6U757)
产品编号 TMAC-01638 别名 RTR, Retinoid receptor-related testis-specific receptor, Retinoid receptor related testis specific receptor, Retinoic acid receptor related testis associated receptor, Orphan nuclear receptor NR6A1, Nuclear receptor subfamily 6 group A member 1, NR6A1_HUMAN, nr6a1, NR61, NR 61, hRTR, hGCNF, Germ cell nuclear factor, GCNF1, GCNF 1, GCNF

Anti-GCNF Antibody (6U757) 是一种 Mouse 抗体，靶向 GCNF。Anti-GCNF Antibody (6U757) 可用于 WB,ICC,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,980
5日内发货
100 μL
¥ 2,995
5日内发货
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产品介绍

生物活性
产品描述
Anti-GCNF Antibody (6U757) is a Mouse antibody targeting GCNF. Anti-GCNF Antibody (6U757) can be used in WB,ICC,IHC,FCM.
别名RTR, Retinoid receptor-related testis-specific receptor, Retinoid receptor related testis specific receptor, Retinoic acid receptor related testis associated receptor, Orphan nuclear receptor NR6A1, Nuclear receptor subfamily 6 group A member 1, NR6A1_HUMAN, nr6a1, NR61, NR 61, hRTR, hGCNF, Germ cell nuclear factor, GCNF1, GCNF 1, GCNF
克隆号
6U757
交叉反应
Human
验证活性
1. Western blot analysis of GCNF on human GCNF recombinant protein using anti- GCNF antibody at 1/1,000 dilution.
2. Western blot analysis of GCNF on HEK293 (1) and GCNF -hIgGFc transfected HEK293 (2) cell lysate using anti-GCNF antibody at 1/1,000 dilution.
3. Western blot analysis of GCNF on different cell lysates using anti-GCNF antibody at 1/1,000 dilution. Positive control:Lane1: K562, Lane2: NTERA-2, Lane3: HEK293, Lane4: HUVE-12, Lane5: HeLa.
4. Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-GCNF antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-GCNF antibody. Counter stained with hematoxylin.
6. ICC staining GCNF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of K562 cells with GCNF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
应用
WB
ICC
IHC
FCM
推荐剂量
WB: 1:500-1000; IHC: 1:100-500; ICC: 1:50-200; FCM: 1:100-200
抗体种类
Monoclonal
宿主来源Mouse
构建方式Hybridoma Monoclonal Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
研究背景Germ cell nuclear factor (GCNF) is an orphan member of the nuclear receptor gene superfamily that influences neurogenesis and germ cell development. GCNF can homodimerize and bind DNA. GCNF regulates paracrine interaction between the oocyte and somatic cells by regulating the expression of BMP-15 and GDF-9, to affect female fertility. GCNF is present in spermatocytes and round spermatids of adult male mouse testis; northern blot and ribonuclease protection assays have shown GCNF is predominant in the testis. The gene expresses three alternatively spliced transcript variants.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q15406
化学信息
分子量Theoretical: 54 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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