Anti-ganglioside GM1 Polyclonal Antibody is a Rabbit antibody targeting ganglioside GM1. Anti-ganglioside GM1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit,GuineaPig)

1. Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-ganglioside GM1/AF647 Conjugated antibody (TMAB-00738-AF647), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647 (orange),used under the same conditions.
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GM1 (GS) Polyclonal Antibody, Unconjugated (TMAB-00738) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2μg/Test

Polyclonal

KLH conjugated ganglioside GM1 purified from pig

Pig

Other Antibodies,Neurodegenerative disease