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Anti-Galectin-3/LGALS3 Antibody (5L119)
还可以产品编号 TMAY-00557
Anti-Galectin-3/LGALS3 Antibody (5L119) 是一种 Mouse 抗体，靶向 Galectin-3/LGALS3。Anti-Galectin-3/LGALS3 Antibody (5L119) 可用于 WB,ELISA,IP。
Anti-Galectin-3/LGALS3 Antibody (5L119)
Anti-Galectin-3/LGALS3 Antibody (5L119)
还可以产品编号 TMAY-00557
Anti-Galectin-3/LGALS3 Antibody (5L119) 是一种 Mouse 抗体，靶向 Galectin-3/LGALS3。Anti-Galectin-3/LGALS3 Antibody (5L119) 可用于 WB,ELISA,IP。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-Galectin-3/LGALS3 Antibody (5L119) is a Mouse antibody targeting Galectin-3/LGALS3. Anti-Galectin-3/LGALS3 Antibody (5L119) can be used in WB,ELISA,IP. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 5L119 |
| 交叉反应 | Human |
| 验证活性 | 1. Galectin3 was immunoprecipitated using: -Lane A:0.5 mg MCF-7 Whole Cell Lysate. -4 µL anti-Galectin3 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Galectin3 mouse monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 30 kDa. -Observed band size: 30 kDa. 2. Anti-Galectin3 mouse monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell Lysate. -Lane B: SW480 Whole Cell Lysate. -Lane C: A431 Whole Cell Lysate. -Lane D: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:28 kDa. -Observed band size:31 kDa |
| 应用 | WB,ELISA,IP |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 1-4 μL/mg of lysate |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Galectin-3 / LGALS3 (rh Galectin-3 / LGALS3; P17931Met1-Ile250). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | LGALS3 (Galectin 3) is a Protein Coding gene. This gene encodes a member of the galectin family of carbohydrate-binding proteins. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. LGALS3 is a beta-galactoside-binding lectin and plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion, and T-cell regulation. LGALS3 has an important role in tumor progression through inhibition of apoptosis. LGALS3 expression is associated with neoplastic transformation and with differentiation of monocytes to macrophages. Elevated expression of LGALS3 has been demonstrated in the synovium of rheumatoid arthritis (RA). Diseases associated with LGALS3 include Follicular Adenoma and Papillary Carcinoma. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human Galectin-3 / LGALS3 protein |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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