Anti-Galectin 3 Antibody (5T518) is a Mouse antibody targeting Galectin 3. Anti-Galectin 3 Antibody (5T518) can be used in WB,ICC/IF.

IgG

5T518

Human

1. 4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Galectin 3) monoclonal Antibody, unconjugated (TMAB-06287) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. 25 μg total protein per lane of various lysates (see on figure) probed with Galectin 3 monoclonal antibody, unconjugated (TMAB-06287) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
3. 25 μg total protein per lane of various lysates (see on figure) probed with Galectin 3 monoclonal antibody, unconjugated (TMAB-06287) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; ICC/IF: 1:100-500

Monoclonal

Recombinant Protein: human Galectin-3 full length protein

Human

LGALS3

Galectin-3

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.