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Anti-GABRA2 Polyclonal Antibody

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Anti-GABRA2 Polyclonal Antibody
产品编号 TMAB-00730

Anti-GABRA2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 GABRA2。Anti-GABRA2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。

Anti-GABRA2 Polyclonal Antibody

Anti-GABRA2 Polyclonal Antibody

Rating icon 还可以
Anti-GABRA2 Polyclonal Antibody
产品编号 TMAB-00730

Anti-GABRA2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 GABRA2。Anti-GABRA2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。

规格价格库存数量
50 μL
¥ 1,170
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-GABRA2 Polyclonal Antibody is a Rabbit antibody targeting GABRA2. Anti-GABRA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
交叉反应
Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit)
验证活性
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GABRA2 Polyclonal Antibody, Unconjugated (TMAB-00730) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Cerebrum (mouse) Lysate at 40 μg
Primary: Anti-GABRA2 (TMAB-00730) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 50 kDa
3. Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-GABRA2 antibody (TMAB-00730), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
4. Sample:
Cerebral cortex (Rat) Lysate at 40 μg
Primary: Anti-GABRA2 (TMAB-00730) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
5. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-GABRA2 (TMAB-00730) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
KLH conjugated synthetic peptide: human GABRA2
抗原种属
Human
基因ID
Uniprot ID
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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