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Anti-GABRA2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 GABRA2。Anti-GABRA2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
Anti-GABRA2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 GABRA2。Anti-GABRA2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,170 | 5日内发货 | |
100 μL | ¥ 1,965 | 5日内发货 | |
200 μL | ¥ 2,780 | 5日内发货 |
产品描述 | Anti-GABRA2 Polyclonal Antibody is a Rabbit antibody targeting GABRA2. Anti-GABRA2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
交叉反应 | Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Rabbit) |
验证活性 | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-GABRA2 Polyclonal Antibody, Unconjugated (TMAB-00730) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Cerebrum (mouse) Lysate at 40 μg Primary: Anti-GABRA2 (TMAB-00730) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 50 kDa 3. Blank control (blue): RSC96 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-GABRA2 antibody (TMAB-00730), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 4. Sample: Cerebral cortex (Rat) Lysate at 40 μg Primary: Anti-GABRA2 (TMAB-00730) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa 5. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-GABRA2 (TMAB-00730) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
免疫原 | KLH conjugated synthetic peptide: human GABRA2 |
抗原种属 | Human |
基因ID | |
Uniprot ID |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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