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顶部生物活性储存和溶解信息计算器

Anti-FUS Antibody (3F162)

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Anti-FUS Antibody (3F162)
产品编号 TMAC-01575
别名 Translocated in liposarcoma protein, TLS, RNA-binding protein FUS, POMp75, Oncogene TLS, Oncogene FUS, FUS, 75 kDa DNA-pairing protein

Anti-FUS Antibody (3F162) 是一种 Rabbit 抗体，靶向 FUS。Anti-FUS Antibody (3F162) 可用于 WB,ICC/IF,IHC,FCM。

Anti-FUS Antibody (3F162)

Anti-FUS Antibody (3F162)

Rating icon 还可以
Anti-FUS Antibody (3F162)
产品编号 TMAC-01575 别名 Translocated in liposarcoma protein, TLS, RNA-binding protein FUS, POMp75, Oncogene TLS, Oncogene FUS, FUS, 75 kDa DNA-pairing protein

Anti-FUS Antibody (3F162) 是一种 Rabbit 抗体，靶向 FUS。Anti-FUS Antibody (3F162) 可用于 WB,ICC/IF,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-FUS Antibody (3F162) is a Rabbit antibody targeting FUS. Anti-FUS Antibody (3F162) can be used in WB,ICC/IF,IHC,FCM.
别名Translocated in liposarcoma protein, TLS, RNA-binding protein FUS, POMp75, Oncogene TLS, Oncogene FUS, FUS, 75 kDa DNA-pairing protein
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of FUS/TLS on K562 cells lysates using anti-FUS/TLS antibody at 1/1,000 dilution.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FUS/TLS antibody. Counter stained with hematoxylin.
3. ICC staining FUS/TLS in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining FUS/TLS in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining FUS/TLS in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. Flow cytometric analysis of Hela cells with FUS/TLS antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IHC
FCM
推荐剂量
WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景EWS and FUS/TLS are nuclear RNA-binding proteins. As a result of chromosome translocation, the EWS gene is fused to a variety of transcription factors, including ATF-1, in human neoplasias. In the Ewing family of tumors, the N-terminal domain of EWS is fused to the DNA-binding domain of various Ets transcription factors, including Fli-1, ETV1 and FEV. The EWS/Fli-1 chimeric protein acts as a more potent transcriptional activator than Fli-1 and can promote cell transformation. In human myxoid liposarcomas and myeloid leukemias, chromosomal translocation results in the fusion of the N-terminal region of FUS/TLS with the open reading frame of CHOP. In normal cells, FUS/TLS binds to the DNA-binding domains of nuclear steroid receptors and is also present in subpopulations of TFIID complexes, indicating a potential role for FUS/TLS in the processing of primary transcripts that are generated in response to hormone-induced transcription.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P35637
化学信息
分子量Theoretical: 75 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

POMp 75

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