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Anti-Flagellin Antibody (3P374)

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Anti-Flagellin Antibody (3P374)
产品编号 TMAY-02860
别名 flaA

Anti-Flagellin Antibody (3P374) 是一种 Rabbit 抗体,靶向 Flagellin。Anti-Flagellin Antibody (3P374) 可用于 ELISA。

Anti-Flagellin Antibody (3P374)
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Anti-Flagellin Antibody (3P374)

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Anti-Flagellin Antibody (3P374)
产品编号 TMAY-02860 别名 flaA

Anti-Flagellin Antibody (3P374) 是一种 Rabbit 抗体,靶向 Flagellin。Anti-Flagellin Antibody (3P374) 可用于 ELISA。

规格价格库存数量
20 μL
¥ 780
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100 μL
¥ 2,294
5日内发货
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产品介绍

生物活性
产品描述
Anti-Flagellin Antibody (3P374) is a Rabbit antibody targeting Flagellin. Anti-Flagellin Antibody (3P374) can be used in ELISA.
别名flaA
Ig Type
Rabbit IgG
克隆号
3P374
交叉反应
Listeria monocytogenes
应用
ELISA
推荐剂量
ELISA: 1:5000-1:10000
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Listeria monocytogenes Listeria monocytogenes flagellin / flaA (TMPY-03465; YP_013330.1; Met1-Ser287).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS
研究背景The role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. Previous studies have documented the ability of this organism to attach to and colonize plant tissue. Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either FlaA, the gene encoding flagellin, or motAB, genes encoding part of the flagellar motor, and tested for both the ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of alfalfa, radish, and broccoli sprouts; however, some of the FlaA mutants showed reduced colonization ability. The best colonizing wild type strain was reduced in colonization on all three sprout types as a result of a FlaA deletion. A mutant in another background was only affected on alfalfa. The third, a poor alfalfa colonizer was not affected in colonization ability by any of the deletions. Fitness of colonization was measured in experiments of competition between mixtures of mutant and parent strains on sprouts. Here the FlaA and motAB mutants of the three strain backgrounds were impaired in fitness of colonization of alfalfa and radish sprouts, and one strain background showed reduced fitness of both mutant types on broccoli sprouts. Together these data indicate a role for flagella for some strains to physically colonize some plants, while the fitness of that colonization is positively affected by motility in almost all cases.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein: Listeria monocytogenes Listeria monocytogenes flagellin / FlaA protein (TMPY-03465)
抗原种属
Listeria monocytogenes
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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