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Anti-FHIT Antibody (5C583)

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产品编号 TMAY-02732

Anti-FHIT Antibody (5C583) 是一种 Rabbit 抗体,靶向 FHIT。Anti-FHIT Antibody (5C583) 可用于 WB,ELISA,ICC/IF。

Anti-FHIT Antibody (5C583)

Anti-FHIT Antibody (5C583)

Rating icon 还可以
产品编号 TMAY-02732

Anti-FHIT Antibody (5C583) 是一种 Rabbit 抗体,靶向 FHIT。Anti-FHIT Antibody (5C583) 可用于 WB,ELISA,ICC/IF。

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产品介绍

生物活性
产品描述
Anti-FHIT Antibody (5C583) is a Rabbit antibody targeting FHIT. Anti-FHIT Antibody (5C583) can be used in WB,ELISA,ICC/IF.
Ig Type
Monoclonal Rabbit IgG
克隆号
5C583
交叉反应
Human
验证活性
1. Immunofluorescence staining of Human FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FHIT monoclonal antibody (1:50) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).

2. Anti-FHIT rabbit monoclonal antibody at 1:100 dilution.

-Lane A: HL-60 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:17 kDa.

-Observed band size:20 kDa
应用
WB,ELISA,ICC/IF
推荐剂量
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式This antibody was obtained from a rabbit immunized with purified, recombinant Human Fragile histidine triad / FHIT (rh Fragile histidine triad / FHIT; TMPY-03398; P49789; Met1-Gln147).
纯化方式Protein A
性状Liquid
缓冲液0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0
研究背景Fragile histidine triad, also known as FHIT, may play a key role in differentiating humans from apes. Fragile histidine triad gene belongs to the histidine triad gene family. It has been shown that fragile histidine triad synergizes with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. Fragile histidine triad gene works as a tumor suppressor as it has been demonstrated in animal studies. The exact molecular function of FHIT is still partially unclear. It also acts as a tumor suppressor of HER2/neu driven breast cancer.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Human Fragile histidine triad / FHIT Protein (TMPY-03398)
抗原种属
Human
研究领域
Tumor Suppressors
存储&运输
储存方式Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式Shipping with blue ice.

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4个月前
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