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Anti-FHIT Antibody (5C583) 是一种 Rabbit 抗体,靶向 FHIT。Anti-FHIT Antibody (5C583) 可用于 WB,ELISA,ICC/IF。
Anti-FHIT Antibody (5C583) 是一种 Rabbit 抗体,靶向 FHIT。Anti-FHIT Antibody (5C583) 可用于 WB,ELISA,ICC/IF。
产品描述 | Anti-FHIT Antibody (5C583) is a Rabbit antibody targeting FHIT. Anti-FHIT Antibody (5C583) can be used in WB,ELISA,ICC/IF. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 5C583 |
交叉反应 | Human |
验证活性 | 1. Immunofluorescence staining of Human FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FHIT monoclonal antibody (1:50) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). 2. Anti-FHIT rabbit monoclonal antibody at 1:100 dilution. -Lane A: HL-60 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:17 kDa. -Observed band size:20 kDa |
应用 | WB,ELISA,ICC/IF |
推荐剂量 | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Fragile histidine triad / FHIT (rh Fragile histidine triad / FHIT; TMPY-03398; P49789; Met1-Gln147). |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
研究背景 | Fragile histidine triad, also known as FHIT, may play a key role in differentiating humans from apes. Fragile histidine triad gene belongs to the histidine triad gene family. It has been shown that fragile histidine triad synergizes with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. Fragile histidine triad gene works as a tumor suppressor as it has been demonstrated in animal studies. The exact molecular function of FHIT is still partially unclear. It also acts as a tumor suppressor of HER2/neu driven breast cancer. |
偶联 | Unconjugated |
免疫原 | Recombinant Human Fragile histidine triad / FHIT Protein (TMPY-03398) |
抗原种属 | Human |
研究领域 | Tumor Suppressors |
储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
运输方式 | Shipping with blue ice. |
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