Anti-FDPS Antibody (8F434) is a Rabbit antibody targeting FDPS. Anti-FDPS Antibody (8F434) can be used in WB,ELISA,FCM,ICC/IF,IP.

Rabbit IgG

8F434

Human

1. Immunofluorescence staining of Human FDPS in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FDPS monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
2. Flow cytometric analysis of Human FDPS expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human FDPS, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
3. Anti-FDPS rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell Lysate.
-Lane B: Hela Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:48 kDa.
-Observed band size:38 kDa.
4. FDPS was immunoprecipitated using:
-Lane A:0.5 mg Hela Whole Cell Lysate.
-Lane B:0.5 mg HepG2 Whole Cell Lysate.
-Lane C:0.5 mg A549 Whole Cell Lysate.
-2 µL anti-FDPS rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-FDPS rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 48 kDa.
-Observed band size: 38 kDa

WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; IP: 1-4 μL/mg of lysate

Monoclonal

Unconjugated

FITC

2

Recombinant Protein: Human FDPS / Farnesyl Diphosphate Synthase protein (TMPY-02654)

Human