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顶部生物活性储存和溶解信息计算器

Anti-FASN Antibody (5Z147)

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Anti-FASN Antibody (5Z147)
产品编号 TMAC-01449
别名 SDR27X1, OA-519, fatty acid synthase, FAS

Anti-FASN Antibody (5Z147) 是一种 Rabbit 抗体，靶向 FASN。Anti-FASN Antibody (5Z147) 可用于 WB,ICC/IF,IHC,IP,FCM。

Anti-FASN Antibody (5Z147)

Anti-FASN Antibody (5Z147)

Rating icon 还可以
Anti-FASN Antibody (5Z147)
产品编号 TMAC-01449 别名 SDR27X1, OA-519, fatty acid synthase, FAS

Anti-FASN Antibody (5Z147) 是一种 Rabbit 抗体，靶向 FASN。Anti-FASN Antibody (5Z147) 可用于 WB,ICC/IF,IHC,IP,FCM。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,490
5日内发货
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产品介绍

生物活性
产品描述
Anti-FASN Antibody (5Z147) is a Rabbit antibody targeting FASN. Anti-FASN Antibody (5Z147) can be used in WB,ICC/IF,IHC,IP,FCM.
别名SDR27X1, OA-519, fatty acid synthase, FAS
Ig Type
IgG
克隆号
5Z147
交叉反应
Human,Mouse,Rat
验证活性
1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.
2. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.
4. ICC staining Fatty Acid Synthase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Fatty Acid Synthase in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Fatty Acid Synthase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining Fatty Acid Synthase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of A549 cells with Fatty Acid Synthase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IHC
IP
FCM
推荐剂量
WB: 1:1000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Fatty acid biosynthesis is mediated by seven catalytic enzymes and an acyl carrier protein (ACP), to which various acyl intermediates are covalently attached. Fatty Acid Synthase (FAS) is the anabolic enzyme that contains the seven unique catalytic sites and mediates the conversion of acetyl-CoA and malonyl-CoA, in the presence of the cofactor NADPH, into long-chain saturated fatty acids, such as palmitate. Human Fatty Acid Synthase cDNA encodes a 2,504 amino acid protein. Catalytically active Fatty Acid Synthase is a homodimer. Human Fatty Acid Synthase mRNA is variably expressed with abundant levels present in brain, lung and liver. Fatty acid synthetic metabolism is abnormally elevated in tumor cells and may support cell growth or survival of malignant cancers.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P49327
化学信息
分子量Theoretical: 273 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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关键词

OA519OA 519

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