Anti-Fas/CD95 Polyclonal Antibody 2 is a Rabbit antibody targeting Fas/CD95. Anti-Fas/CD95 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Pig)

1. Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-CD95/FAS antibody (TMAB-00657)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Blank control (blue): Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-CD95/FAS antibody (TMAB-00657,Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
3. Sample:
Lane 1: Thymus (Mouse) Lysate at 40 μg
Lane 2: Thymus (Rat) Lysate at 40 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: Raji (Human) Cell Lysate at 30 μg
Lane 5: HT1080 (Human) Cell Lysate at 30 μg
Lane 6: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-CD95/FAS (TMAB-00657) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45/52 kDa
Observed band size: 52 kDa
4. Sample: serum (Rat) at 40 μg plasma (Rat) at 40 μg
Primary: Anti-CD95 (TMAB-00657) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 42 kDa

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human FAS/Apo-1/CD95

Human

Death Receptors,Cytokines,TNF Superfamily