Anti-Fas/CD95 Polyclonal Antibody is a Rabbit antibody targeting Fas/CD95. Anti-Fas/CD95 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Pig)

1. Sample:
Lane 1: Thymus (Mouse) Lysate at 40 μg
Lane 2: Thymus (Rat) Lysate at 40 μg
Lane 3: HepG2 (Human) Cell Lysate at 30 μg
Lane 4: Raji (Human) Cell Lysate at 30 μg
Lane 5: HT1080 (Human) Cell Lysate at 30 μg
Lane 6: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-CD95/FAS (TMAB-00656) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45/52 kDa
Observed band size: 52 kDa
2. Sample: serum (Rat) at 40 μg plasma (Rat) at 40 μg
Primary: Anti-CD95 (TMAB-00656) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 42 kDa
3. Blank control (blue): Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37°C). Primary Antibody: Rabbit Anti-CD95/FAS antibody (TMAB-00656,Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
4. Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-CD95/FAS antibody (TMAB-00656)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: rat FAS

Rat

FAS

Tumor necrosis factor receptor superfamily member 6

Death Receptors,Cytokines,TNF Superfamily

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).