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Anti-Fas/CD95 Antibody (4A58)
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产品编号 TMAC-01446
别名 TNFRSF6, FASTM, FAS1, Fas cell surface death receptor, CD95, APT1, APO-1, ALPS1A
Anti-Fas/CD95 Antibody (4A58) 是一种 Rabbit 抗体，靶向 Fas/CD95。Anti-Fas/CD95 Antibody (4A58) 可用于 WB,ICC/IF,IHC,FCM。
Anti-Fas/CD95 Antibody (4A58)
Anti-Fas/CD95 Antibody (4A58)
还可以产品编号 TMAC-01446 别名 TNFRSF6, FASTM, FAS1, Fas cell surface death receptor, CD95, APT1, APO-1, ALPS1A
Anti-Fas/CD95 Antibody (4A58) 是一种 Rabbit 抗体，靶向 Fas/CD95。Anti-Fas/CD95 Antibody (4A58) 可用于 WB,ICC/IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,495 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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| 产品描述 | Anti-Fas/CD95 Antibody (4A58) is a Rabbit antibody targeting Fas/CD95. Anti-Fas/CD95 Antibody (4A58) can be used in WB,ICC/IF,IHC,FCM. |
| 别名 | TNFRSF6, FASTM, FAS1, Fas cell surface death receptor, CD95, APT1, APO-1, ALPS1A |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fas antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fas antibody. Counter stained with hematoxylin. 3. ICC staining Fas in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Fas in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of Raji cells with Fas antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immuno-surveillance against virus-infected or transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FAS-based mechanism involves the transducing molecule FAS (also designated APO-1) and its ligand (FAS-L). The human FAS protein is a cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of which undergo apoptosis in response to treatment with antibody to FAS. These findings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FAS may be critically involved in CTL-mediated cytotoxicity. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 45 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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