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顶部生物活性储存和溶解信息计算器

Anti-ERK5 Antibody (5E286)

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Anti-ERK5 Antibody (5E286)
产品编号 TMAC-01373
别名 PROTEIN KINASE, MITOGEN-ACTIVATED, 7, PRKM7, PRKM 7, OTTHUMP00000065907, OTTHUMP00000065906, MK07, Mitogen-activated protein kinase 7, Mitogen Activated Protein Kinase 7, MAPK7, MAPK 7, MAP kinase 7, Extracellular signal-regulated kinase 5, Extracellular Signal Regulated Kinase 5, ERK4, ERK 5, ERK 4, EC 2.7.11.24, BMK1 Kinase, BMK1, BMK 1 kinase, BMK 1, Big MAP kinase 1

Anti-ERK5 Antibody (5E286) 是一种 Rabbit 抗体，靶向 ERK5。Anti-ERK5 Antibody (5E286) 可用于 WB,ICC/IF,IP,FCM。

Anti-ERK5 Antibody (5E286)

Anti-ERK5 Antibody (5E286)

Rating icon 还可以
Anti-ERK5 Antibody (5E286)
产品编号 TMAC-01373 别名 PROTEIN KINASE, MITOGEN-ACTIVATED, 7, PRKM7, PRKM 7, OTTHUMP00000065907, OTTHUMP00000065906, MK07, Mitogen-activated protein kinase 7, Mitogen Activated Protein Kinase 7, MAPK7, MAPK 7, MAP kinase 7, Extracellular signal-regulated kinase 5, Extracellular Signal Regulated Kinase 5, ERK4, ERK 5, ERK 4, EC 2.7.11.24, BMK1 Kinase, BMK1, BMK 1 kinase, BMK 1, Big MAP kinase 1

Anti-ERK5 Antibody (5E286) 是一种 Rabbit 抗体，靶向 ERK5。Anti-ERK5 Antibody (5E286) 可用于 WB,ICC/IF,IP,FCM。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-ERK5 Antibody (5E286) is a Rabbit antibody targeting ERK5. Anti-ERK5 Antibody (5E286) can be used in WB,ICC/IF,IP,FCM.
别名PROTEIN KINASE, MITOGEN-ACTIVATED, 7, PRKM7, PRKM 7, OTTHUMP00000065907, OTTHUMP00000065906, MK07, Mitogen-activated protein kinase 7, Mitogen Activated Protein Kinase 7, MAPK7, MAPK 7, MAP kinase 7, Extracellular signal-regulated kinase 5, Extracellular Signal Regulated Kinase 5, ERK4, ERK 5, ERK 4, EC 2.7.11.24, BMK1 Kinase, BMK1, BMK 1 kinase, BMK 1, Big MAP kinase 1
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of ERK5 on Hela cells lysates using anti-ERK5 antibody at 1/1,000 dilution.
2. ICC staining ERK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining ERK5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining ERK5 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. Flow cytometric analysis of A549 cells with ERK5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IP
FCM
推荐剂量
WB: 1:1000-2000; ICC/IF: 1:100-500; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q13164
化学信息
分子量Theoretical: 115 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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