Anti-ERK1/2 Polyclonal Antibody 2 is a Rabbit antibody targeting ERK1/2. Anti-ERK1/2 Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,Goat)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (TMAB-00634) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (TMAB-00634) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
3. Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated (TMAB-00634) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
4. Sample:
Lane 1: A431 (Human) Cell Lysate at 30 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Rat) Lysate at 40 μg
Lane 4: LympHnode (Mouse) Lysate at 40 μg
Lane 5: LympHnode (Rat) Lysate at 40 μg
Lane 6: SiHa (Human) Cell Lysate at 30 μg
Lane 7: HUVEC (Human) Cell Lysate at 30 μg
Primary: Anti-ERK1 + ERK2 (TMAB-00634) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44/42 kDa
Observed band size: 38 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ERK1/2

Human

Tangles & Tau,MAPK Pathway,Cytoplasmic