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Anti-EPN1 Antibody (4A473) 是一种 Mouse 抗体,靶向 EPN1。Anti-EPN1 Antibody (4A473) 可用于 WB,FCM。
Anti-EPN1 Antibody (4A473) 是一种 Mouse 抗体,靶向 EPN1。Anti-EPN1 Antibody (4A473) 可用于 WB,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,995 | 5日内发货 | |
100 μL | ¥ 2,990 | 5日内发货 |
产品描述 | Anti-EPN1 Antibody (4A473) is a Mouse antibody targeting EPN1. Anti-EPN1 Antibody (4A473) can be used in WB,FCM. |
别名 | Erythropoietin receptor, EPO-R, Epor |
交叉反应 | Human |
验证活性 | 1. Western blot analysis of EPN1 on human EPN1 recombinant protein using anti-EPN1 antibody at 1/1,000 dilution. 2. Western blot analysis of EPN1 on HEK293 (1) and EPN1-hIgGFc transfected HEK293 (2) cell lysate using anti-EPN1 antibody at 1/1,000 dilution. 3. Flow cytometric analysis of A431 cells with EPN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
应用 | |
推荐剂量 | WB: 1:500-2000; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Mouse |
构建方式 | Hybridoma Monoclonal Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Epsin 1 (EPN1) is an endocytic accessory protein, with significant similarity to the Xenopus mitotic phosphoprotein MP90. Epsin 1 interacts with Eps15 (the 伪 subunit of the Clathrin adaptor AP2), Clathrin and other accessory proteins. The mitotic phosphorylation of these proteins may be one of the mechanisms by which the invagination of Clathrin-coated pits is blocked in mitosis. Both epsin and Eps15, like other cytosolic components of the synaptic vesicle endocytic machinery, undergo constitutive phosphorylation and depolarization-dependent dephosphorylation in nerve terminals. Epsin 1 also contributes to the mechanism of Clathrin-vesicle-dependent endocytosis. The human Epsin 1 protein contains an epsin N-terminal homology (ENTH) region and a single Clathrin-binding (LVDLD) motif. Epsin 1 localizes to the leading edge of a vesicular coated pit where the membrane is being actively bent. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 60 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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