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Anti-EphB4 Antibody-PE (6A752)
还可以产品编号 TMAY-00504P
Anti-EphB4 Antibody-PE (6A752) 是一种 PE-conjugated Mouse 抗体，靶向 EphB4。Anti-EphB4 Antibody-PE (6A752) 可用于 FCM。
Anti-EphB4 Antibody-PE (6A752)
Anti-EphB4 Antibody-PE (6A752)
还可以产品编号 TMAY-00504P
Anti-EphB4 Antibody-PE (6A752) 是一种 PE-conjugated Mouse 抗体，靶向 EphB4。Anti-EphB4 Antibody-PE (6A752) 可用于 FCM。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-EphB4 Antibody-PE (6A752) is a PE-conjugated Mouse antibody targeting EphB4. Anti-EphB4 Antibody-PE (6A752) can be used in FCM. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 6A752 |
| 交叉反应 | Human |
| 验证活性 | 1. Profile of anti-EphB4 reactivity on MCF-7 cells analyzed by flow cytometry. 2. Immunofluorescence staining of Human EphB4 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human EphB4 monoclonal antibody (PE-conjugated, 10 µg/ml) at 37°C 1 hour. Positive staining was localized to plasma membrane. |
| 应用 | FCM |
| 推荐剂量 | 5 μl/Test, 0.1 mg/ml |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EphB4 (rh EphB4; TMPY-00787; NP_004435.3; Met 1-Ala 539) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | PBS solution containing 0.5% BSA and 0.09% sodium azide |
| 研究背景 | Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers. |
| 偶联 | PE |
| 其他可选修饰符 | Unconjugated/APC/FITC |
| 可提供抗体种类 | 4 |
| 免疫原 | Recombinant Human EphB4 protein (TMPY-00787) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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