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Anti-EphB3 Antibody-APC (8R580) 是一种 APC-conjugated Rabbit 抗体,靶向 EphB3。Anti-EphB3 Antibody-APC (8R580) 可用于 FCM。
Anti-EphB3 Antibody-APC (8R580) 是一种 APC-conjugated Rabbit 抗体,靶向 EphB3。Anti-EphB3 Antibody-APC (8R580) 可用于 FCM。
产品描述 | Anti-EphB3 Antibody-APC (8R580) is a APC-conjugated Rabbit antibody targeting EphB3. Anti-EphB3 Antibody-APC (8R580) can be used in FCM. |
Ig Type | Monoclonal Rabbit IgG |
克隆号 | 8R580 |
交叉反应 | Mouse |
验证活性 | Flow cytometric analysis of Mouse EphB3 expression on CoLo205 cells. Cells were stained with APC-conjugated anti-Mouse Mouse EphB3. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
应用 | FCM |
推荐剂量 | 10 μl/Test, 0.1 mg/ml |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse EphB3 / HEK2 (rM EphB3 / HEK2; TMPY-01784; NP_034273.1; Met1-Thr537) and conjugated with APC under optimum conditions, the unreacted APC was removed. |
纯化方式 | Protein A |
性状 | Liquid |
缓冲液 | PBS solution containing 0.5% BSA and 0.09% sodium azide |
研究背景 | Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB. |
偶联 | APC |
其他可选修饰符 | Unconjugated/PE |
可提供抗体种类 | 3 |
免疫原 | Recombinant Mouse EphB3 / HEK2 protein (TMPY-01784) |
抗原种属 | Mouse |
储存方式 | Store at 2°C-8°C for 12 months, do not freeze. Keep away from direct sunlight. Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
运输方式 | Shipping with blue ice. |
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