Anti-ENO1 Antibody (8H69)

Name: Anti-ENO1 Antibody (8H69)
Brand: TargetMol
SKU: TMAH-00386
Rating: 4.5 (1 reviews)

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产品编号 TMAH-00386

别名 PPH, NNE, MPB1, HEL-S-17, enolase 1, (α), enolase 1, (alpha), ENO1L1

Anti-ENO1 Antibody (8H69) 是一种 Mouse 抗体，靶向 ENO1。Anti-ENO1 Antibody (8H69) 可用于 ELISA, WB, IF, FCM, IP。

Anti-ENO1 Antibody (8H69)

还可以

产品编号 TMAH-00386 别名 PPH, NNE, MPB1, HEL-S-17, enolase 1, (α), enolase 1, (alpha), ENO1L1

Anti-ENO1 Antibody (8H69) 是一种 Mouse 抗体，靶向 ENO1。Anti-ENO1 Antibody (8H69) 可用于 ELISA, WB, IF, FCM, IP。

规格	价格	库存	数量
50 μL	¥ 1,315	5日内发货
100 μL	¥ 2,190	5日内发货

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产品描述	Anti-ENO1 Antibody (8H69) is a Mouse antibody targeting ENO1. Anti-ENO1 Antibody (8H69) can be used in ELISA, WB, IF, FCM, IP.
别名	PPH, NNE, MPB1, HEL-S-17, enolase 1, (α), enolase 1, (alpha), ENO1L1
Ig Type	IgG1
克隆号	8H69
交叉反应	Human, Mouse, Rat, Rabbit
验证活性	1. Western Blot -Positive WB detected in: K562 whole cell lysate, Rabbit Skeletal Muscle tissue, Rabbit Kidney lysate -All lanes ENO1 antibody at 1:10000 -Secondary: Goat polyclonal to mouse IgG at 1/10000 dilution -Predicted band size: 47 KDa -Observed band size: 47 KDa -Exposure time: 1min 2. Western Blot -Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate -All lanes ENO1 antibody at 1:10000 -Secondary: Goat polyclonal to mouse IgG at 1/10000 dilution -Predicted band size: 47 KDa -Observed band size: 47 KDa -Exposure time: 10s 3. Western Blot -Positive WB detected in: HepG2 whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg -All lanes: ENO1 antibody at 1:5000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 47 kDa -Observed band size: 47 KDa -Exposure time: 10s 4. Western Blot -Positive WB detected in: MCF-7 whole cell lysate -All lanes: ENO1 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 47 kDa -Observed band size: 47 KDa -Exposure time: 10s 5. Immunofluorescence staining of MCF-7 cells with TMAH-00386 at 1:270, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 6. Immunofluorescence staining of Hela cells with TMAH-00386 at 1:270, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 7. Overlay histogram showing MCF-7 cells stained with TMAH-00386 (red line) at 1:550. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. 8. Overlay histogram showing Hela cells stained with TMAH-00386 (red line) at 1:550. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. 9. Immunoprecipitating ENO1 in HepG2 whole cell lysate -Lane 1: Mouse control IgG (1µg) instead of TMAH-00386 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00386 (1µl) + HepG2 whole cell lysate (500µg) -Lane 3: HepG2 whole cell lysate (10µg)
应用	ELISA, WB, IF, FCM, IP
抗体种类	Monoclonal
宿主来源	Mouse
亚细胞定位	Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.; [Isoform MBP-1]: Nucleus.
构建方式	Hybridoma Monoclonal Antibody
纯化方式	Protein G purified
性状	Liquid
缓冲液	Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
纯度	>95%
研究背景	Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.