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Anti-Engrailed 1 Polyclonal Antibody
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产品编号 TMAB-00613
Anti-Engrailed 1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Engrailed 1。Anti-Engrailed 1 Polyclonal Antibody 可用于 WB,FCM。
Anti-Engrailed 1 Polyclonal Antibody
Anti-Engrailed 1 Polyclonal Antibody
还可以产品编号 TMAB-00613
Anti-Engrailed 1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 Engrailed 1。Anti-Engrailed 1 Polyclonal Antibody 可用于 WB,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,165 | 5日内发货 | |
| 100 μL | ¥ 1,965 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-Engrailed 1 Polyclonal Antibody is a Rabbit antibody targeting Engrailed 1. Anti-Engrailed 1 Polyclonal Antibody can be used in WB,FCM. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Sheep) |
| 验证活性 | 1. Blank control (blue): Raji Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Engrailed 1/AF647 Conjugated antibody (TMAB-00613-AF647), Dilution: 0.2 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC (orange),used under the same conditions. 2. Sample: Lane 1: Muscle (Mouse) Lysate at 40 μg Lane 2: Cerebrum (Mouse) Lysate at 40 μg Lane 3: Muscle (Rat) Lysate at 40 μg Lane 4: Cerebrum (Rat) Lysate at 40 μg Primary: Anti-Engrailed 1 (TMAB-00613) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 50 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; FCM: 1μg/Test |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Nucleus. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | The engrailed-1 gene, EN1, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homo-logs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expres-sion of a-synuclein, a gene that is genetically linked to Parkinson’s disease. |
| 免疫原 | KLH conjugated synthetic peptide: human EN1/Engrailed 1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Nuclear,Ectoderm,Neurogenesis,Other factors,Developmental Families,Ectoderm |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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