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Anti-ELMO1 Antibody (8W516)
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产品编号 TMAC-01287
别名 Protein ced-12 homolog, MGC126406, KIAA0281, Engulfment and cell motility protein 1, Engulfment and cell motility 1, ELMO1_HUMAN, ELMO-1, Elmo1, ELMO 1, Ced12 homolog 1, Ced12 homolog, CED-12, CED12, Ced 12 homolog 1, Ced 12 homolog, CED 12
Anti-ELMO1 Antibody (8W516) 是一种 Rabbit 抗体，靶向 ELMO1。Anti-ELMO1 Antibody (8W516) 可用于 WB,IHC,FCM,IP。
Anti-ELMO1 Antibody (8W516)
Anti-ELMO1 Antibody (8W516)
还可以产品编号 TMAC-01287 别名 Protein ced-12 homolog, MGC126406, KIAA0281, Engulfment and cell motility protein 1, Engulfment and cell motility 1, ELMO1_HUMAN, ELMO-1, Elmo1, ELMO 1, Ced12 homolog 1, Ced12 homolog, CED-12, CED12, Ced 12 homolog 1, Ced 12 homolog, CED 12
Anti-ELMO1 Antibody (8W516) 是一种 Rabbit 抗体，靶向 ELMO1。Anti-ELMO1 Antibody (8W516) 可用于 WB,IHC,FCM,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-ELMO1 Antibody (8W516) is a Rabbit antibody targeting ELMO1. Anti-ELMO1 Antibody (8W516) can be used in WB,IHC,FCM,IP. |
| 别名 | Protein ced-12 homolog, MGC126406, KIAA0281, Engulfment and cell motility protein 1, Engulfment and cell motility 1, ELMO1_HUMAN, ELMO-1, Elmo1, ELMO 1, Ced12 homolog 1, Ced12 homolog, CED-12, CED12, Ced 12 homolog 1, Ced 12 homolog, CED 12 |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of ELMO1 on mouse spleen tissue and Jurkat cell lysates using anti-ELMO1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-ELMO1 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. 3. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-ELMO1 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. 4. Flow cytometric analysis of SH-SY-5Y cells with ELMO1 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; IP: 1:50-100; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | Elmo (engulfment and cell motility) proteins share similarity to C. elegans CED-12. The C. elegans genes ced-2, ced-5, ced-10 and ced-12, and their mammalian homologs, CRKII, DOCK1, RAC1 and ELMO, mediate cytoskeletal rear-rangements during phagocytosis of apoptotic cells as well as cell motility. Elmo1 associates with DOCK 180 and may influence phagocytosis and effect cell shape changes. Src family kinase-mediated tyrosine phosphorylation of Elmo1 influences signaling through Elmo1/Crk/DOCK 180 pathways. Elmo2 interacts directly with Rho G in a GTP-dependent manner and forms a ternary complex with DOCK 180 to induce activation of Rac 1. The Rho G-Elmo2-DOCK 180 pathway is required for activation of Rac 1 and cell spreading mediated by integrin, as well as for neurite outgrowth induced by nerve growth factor. Elmo3 acts in assocation with DOCK 180 and Crk II and may be required in complex with DOCK 180 to activate Rac/Rho small GTPases. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: human ELMO1 aa 500 to 700 |
| 抗原种属 | human |
| Uniprot ID |
| 分子量 | Theoretical: 84 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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