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Anti-Elastin Polyclonal Antibody 2

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Anti-Elastin Polyclonal Antibody 2
产品编号 TMAB-00605

Anti-Elastin Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 Elastin。Anti-Elastin Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF,FCM。

Anti-Elastin Polyclonal Antibody 2

Anti-Elastin Polyclonal Antibody 2

Rating icon 还可以
Anti-Elastin Polyclonal Antibody 2
产品编号 TMAB-00605

Anti-Elastin Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 Elastin。Anti-Elastin Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF,FCM。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-Elastin Polyclonal Antibody 2 is a Rabbit antibody targeting Elastin. Anti-Elastin Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. The figure annotation: The blue histogram is unstained cells. The green histogram is cells stained with Rabbit Anti-alpha Elastin/Tropoelastin antibody (TMAB-00605) plus secondary antibody.
Controls: Positive control: A549 cells. Isotype control: Cell lines treated with rabbit IgG at 1:1000 4°C temperature overnight 4°Cfollowed by conjugation to secondary antibody.
3. Sample: Lung (Mouse) Lysate at 30 μg
Primary: Anti-alpha Elastin/Tropoelastin (TMAB-00605) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 70 kDa
Observed band size: 68 kDa
4. Sample:
Lane 1: Uterus (Mouse) Lysate at 40 μg
Lane 2: Lung (Mouse) Lysate at 40 μg
Lane 3: Liver (Mouse) Lysate at 40 μg
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 5: Lung (Rat) Lysate at 40 μg
Lane 6: Liver (Rat) Lysate at 40 μg
Lane 7: Testis (Rat) Lysate at 40 μg
Lane 8: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 9: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Elastin (TMAB-00605) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 68 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景This gene encodes a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Degradation products of the encoded protein, known as elastin-derived peptides or elastokines, bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. [provided by RefSeq, Aug 2017].
抗原信息
免疫原
KLH conjugated synthetic peptide: human Elastin
抗原种属
Human
基因ID
Uniprot ID
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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