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Anti-Elastin Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 Elastin。Anti-Elastin Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
Anti-Elastin Polyclonal Antibody 2 是一种 Rabbit 抗体,靶向 Elastin。Anti-Elastin Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,165 | 5日内发货 | |
100 μL | ¥ 1,970 | 5日内发货 | |
200 μL | ¥ 2,795 | 5日内发货 |
产品描述 | Anti-Elastin Polyclonal Antibody 2 is a Rabbit antibody targeting Elastin. Anti-Elastin Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. The figure annotation: The blue histogram is unstained cells. The green histogram is cells stained with Rabbit Anti-alpha Elastin/Tropoelastin antibody (TMAB-00605) plus secondary antibody. Controls: Positive control: A549 cells. Isotype control: Cell lines treated with rabbit IgG at 1:1000 4°C temperature overnight 4°Cfollowed by conjugation to secondary antibody. 3. Sample: Lung (Mouse) Lysate at 30 μg Primary: Anti-alpha Elastin/Tropoelastin (TMAB-00605) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 70 kDa Observed band size: 68 kDa 4. Sample: Lane 1: Uterus (Mouse) Lysate at 40 μg Lane 2: Lung (Mouse) Lysate at 40 μg Lane 3: Liver (Mouse) Lysate at 40 μg Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 5: Lung (Rat) Lysate at 40 μg Lane 6: Liver (Rat) Lysate at 40 μg Lane 7: Testis (Rat) Lysate at 40 μg Lane 8: SH-SY5Y (Human) Cell Lysate at 30 μg Lane 9: A549 (Human) Cell Lysate at 30 μg Primary: Anti-Elastin (TMAB-00605) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kDa Observed band size: 68 kDa |
应用 | |
推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | This gene encodes a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein is rich in hydrophobic amino acids such as glycine and proline, which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Degradation products of the encoded protein, known as elastin-derived peptides or elastokines, bind the elastin receptor complex and other receptors and stimulate migration and proliferation of monocytes and skin fibroblasts. Elastokines can also contribute to cancer progression. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. [provided by RefSeq, Aug 2017]. |
免疫原 | KLH conjugated synthetic peptide: human Elastin |
抗原种属 | Human |
基因ID | |
Uniprot ID |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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