Anti-Elastin Polyclonal Antibody 2 is a Rabbit antibody targeting Elastin. Anti-Elastin Polyclonal Antibody 2 can be used in FCM,IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse,Rat

1. Images provided the Independent Validation Program (badge number 28751): Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody (TMAB-00605) at 1:1000 4℃ temperature overnight 4℃followed by conjugation to secondary antibody.
2. Sample: Lung (Mouse) Lysate at 30 μg
Primary: Anti-alpha Elastin/Tropoelastin (TMAB-00605) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 70 kDa
Observed band size: 68 kDa
3. Sample:
Lane 1: Uterus (Mouse) Lysate at 40 μg
Lane 2: Lung (Mouse) Lysate at 40 μg
Lane 3: Liver (Mouse) Lysate at 40 μg
Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 5: Lung (Rat) Lysate at 40 μg
Lane 6: Liver (Rat) Lysate at 40 μg
Lane 7: Testis (Rat) Lysate at 40 μg
Lane 8: SH-SY5Y (Human) Cell Lysate at 30 μg
Lane 9: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-Elastin (TMAB-00605) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 68 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: human Elastin

Human

ELN

Elastin

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.