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顶部生物活性储存和溶解信息计算器

Anti-EIF5A Antibody (9P939)

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产品编号 TMAC-01281

别名 eukaryotic translation initiation factor 5A, eIF5AI, EIF5A1, EIF-5A

Anti-EIF5A Antibody (9P939) 是一种 Rabbit 抗体，靶向 EIF5A。Anti-EIF5A Antibody (9P939) 可用于 FCM,ICC,IHC,IP,WB。

Anti-EIF5A Antibody (9P939)

Anti-EIF5A Antibody (9P939)

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Rating icon 还可以
产品编号 TMAC-01281 别名 eukaryotic translation initiation factor 5A, eIF5AI, EIF5A1, EIF-5A

Anti-EIF5A Antibody (9P939) 是一种 Rabbit 抗体，靶向 EIF5A。Anti-EIF5A Antibody (9P939) 可用于 FCM,ICC,IHC,IP,WB。

规格价格库存数量
50 μL
¥ 1,490
5日内发货
100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-EIF5A Antibody (9P939) is a Rabbit antibody targeting EIF5A. Anti-EIF5A Antibody (9P939) can be used in FCM,ICC,IHC,IP,WB.
别名eukaryotic translation initiation factor 5A, eIF5AI, EIF5A1, EIF-5A
Ig Type
IgG
克隆号
9P939
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of eIF5A on different lysates using anti-eIF5A antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: MCF-7.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-eIF5A antibody. Counter stained with hematoxylin.
5. ICC staining eIF5A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining eIF5A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Jurkat cells with eIF5A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
verifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivityverifiedActivity
应用FCMICCIHCIPWB
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC: 1:100-500; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors. The human eukaryotic translation initiation factor 5A gene, also designated eIF-4D or eIF5A, maps to chromosome 17p13.1 and encodes a 154 amino acid protein that is linked to cellular polyamine homeostasis. eIF5A localizes to the nuclear and cytoplasmic compartments of mammalian cells where it can stimulate ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport and/or protein translation. eIF5A contains a unique spermidine-derived post-translational modification at Lys-50, hypusine, which is necessary for eIF5A's biochemical activity and for cellular proliferative signaling. In addition, eIF5A is a cellular cofactor for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1). Inhibition of eIF5A interaction with Rev leads to a block of the viral replication cycle.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
P63241
化学信息
分子量Theoretical: 18 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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