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Anti-EIF2S1 Antibody (9P904)
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产品编号 TMAC-01263
别名 IF2A_HUMAN, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, EIF2A, EIF2 alpha, EIF2, EIF 2alpha, EIF 2A, EIF 2 alpha, EIF 2
Anti-EIF2S1 Antibody (9P904) 是一种 Mouse 抗体，靶向 EIF2S1。Anti-EIF2S1 Antibody (9P904) 可用于 WB,ICC,IHC,FCM。
Anti-EIF2S1 Antibody (9P904)
Anti-EIF2S1 Antibody (9P904)
还可以产品编号 TMAC-01263 别名 IF2A_HUMAN, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, EIF2A, EIF2 alpha, EIF2, EIF 2alpha, EIF 2A, EIF 2 alpha, EIF 2
Anti-EIF2S1 Antibody (9P904) 是一种 Mouse 抗体，靶向 EIF2S1。Anti-EIF2S1 Antibody (9P904) 可用于 WB,ICC,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,985 | 5日内发货 | |
| 100 μL | ¥ 2,980 | 5日内发货 |
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偶联与修饰
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化学信息
| 产品描述 | Anti-EIF2S1 Antibody (9P904) is a Mouse antibody targeting EIF2S1. Anti-EIF2S1 Antibody (9P904) can be used in WB,ICC,IHC,FCM. |
| 别名 | IF2A_HUMAN, Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa, EIF2A, EIF2 alpha, EIF2, EIF 2alpha, EIF 2A, EIF 2 alpha, EIF 2 |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of EIF2A on human EIF2A recombinant protein using anti-EIF2A antibody at 1/1,000 dilution. 2. Western blot analysis of EIF2A on HEK293 (1) and EIF2A-hIgGFc transfected HEK293 (2) cell lysate using anti-EIF2A antibody at 1/1,000 dilution. 3. Western blot analysis of EIF2A on different cell lysate using anti-EIF2A antibody at 1/1,000 dilution. Positive control: Line1: MCF-7, Line2: PC-12, Line3: HepG2, Line4: Hela, Line5: Cos7, Line6: K562, Line7: Jurkat, Line8: A431, Line9: 4. Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-EIF2A antibody. Counter stained with hematoxylin. 5. ICC staining EIF2A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of HepG2 cells with EIF2A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | eIF2A (eukaryotic translation initiation factor 2A), also known as CDA02, MSTP004 or MSTP089, is a 585 amino acid protein that contains three WD repeats. Expressed ubiquitously with highest expression in heart, brain, pancreas and placenta, eIF2A functions as a translation initiation factor that binds Met-tRNA and directs it to 40S ribosomal subunits. Present in the early steps of protein synthesis, eIF2A controls the binding of Met-tRNA to 40S ribosomal subunits in a codon-dependent manner, in contrast to the eIF2 complex which accomplishes the same task in a GTP-dependent manner. In addition to its role in transcription initiation, eIF2A may also act to negatively regulate the expression of specific proteins, suggesting a possible role as a transcriptional repressor. eIF2A exists as two isoforms due to alternative splicing events. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 65 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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