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Anti-EGFR Antibody (5D853)
还可以产品编号 TMAY-00022
Anti-EGFR Antibody (5D853) 是一种 Mouse 抗体，靶向 EGFR。Anti-EGFR Antibody (5D853) 可用于 FCM,ICC/IF。
Anti-EGFR Antibody (5D853)
Anti-EGFR Antibody (5D853)
还可以产品编号 TMAY-00022
Anti-EGFR Antibody (5D853) 是一种 Mouse 抗体，靶向 EGFR。Anti-EGFR Antibody (5D853) 可用于 FCM,ICC/IF。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-EGFR Antibody (5D853) is a Mouse antibody targeting EGFR. Anti-EGFR Antibody (5D853) can be used in FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 5D853 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of EGFR in A431 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with mouse anti-Human EGFR monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane. 2. Flow cytometric analysis of Human EGFR expression on A431 cells. Cells were stained with purified anti-Human EGFR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/FITC/Biotin/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Human EGFR / HER1 / ErbB1 protein (TMPY-00742) |
| 抗原种属 | Human |
| 研究领域 | Cancer Drug Targets, Receptor Tyrosine Kinases (RTKs) |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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