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Anti-EEF1A1 Antibody (9F149)
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产品编号 TMAC-01230
别名 Eukaryotic elongation factor 1 A-1 (eEF1A-1), Elongation factor Tu (EF-Tu), Elongation factor 1-alpha 1, EF-1-alpha-1, EEF1A1, EEF1A
Anti-EEF1A1 Antibody (9F149) 是一种 Rabbit 抗体，靶向 EEF1A1。Anti-EEF1A1 Antibody (9F149) 可用于 WB,ICC,IF,IHC,FCM,IP。
Anti-EEF1A1 Antibody (9F149)
Anti-EEF1A1 Antibody (9F149)
还可以产品编号 TMAC-01230 别名 Eukaryotic elongation factor 1 A-1 (eEF1A-1), Elongation factor Tu (EF-Tu), Elongation factor 1-alpha 1, EF-1-alpha-1, EEF1A1, EEF1A
Anti-EEF1A1 Antibody (9F149) 是一种 Rabbit 抗体，靶向 EEF1A1。Anti-EEF1A1 Antibody (9F149) 可用于 WB,ICC,IF,IHC,FCM,IP。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,485 | 5日内发货 | |
| 100 μL | ¥ 2,485 | 5日内发货 |
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| 产品描述 | Anti-EEF1A1 Antibody (9F149) is a Rabbit antibody targeting EEF1A1. Anti-EEF1A1 Antibody (9F149) can be used in WB,ICC,IF,IHC,FCM,IP. |
| 别名 | Eukaryotic elongation factor 1 A-1 (eEF1A-1), Elongation factor Tu (EF-Tu), Elongation factor 1-alpha 1, EF-1-alpha-1, EEF1A1, EEF1A |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of eEF1A1 on different lysates using anti-eEF1A1 antibody at 1/500 dilution. Positive control: Lane 1: Rat brain tissue, Lane 2: Mouse skeletal muscle, Lane 3: Mouse cerebellum, Lane 4: Rat skin, Lane 5: Daudi. 2. Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-eEF1A1 antibody. Counter stained with hematoxylin. 7. ICC staining eEF1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining eEF1A1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining eEF1A1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of THP-1 cells with eEF1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The elongation factor-1 complex is composed of two subunits, EF-1 α1 (elongation factor 1-alpha 1) and EF-1 α2 (elongation factor 1-alpha 2), and is responsible for the delivery of aminoacyl tRNAs to the ribosome. EF-1 α1 is expressed predominately in brain, placenta, lung, liver, kidney and pancreas, while EF-1 α2 is highly expressed in heart, brain and skeletal muscle. Both EF-1 α1 and α2 localize to the nucleus and belong to the GTP-binding elongation factor family. The gene encoding EF-1 α2, which maps to human chromosome 20q13.3, may play a role in the development of ovarian cancer, while the EF-1 α1 gene, mapping to chromosome 6Q14.1, is commonly present as an autoantigen in patients with Felty syndrome. Felty syndrome is a disorder characterized by rheumatoid arthritis, a swollen spleen, decreased white blood cell count, and increased susceptibility to infection. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 50 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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