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Anti-DUSP1 Antibody (4S873) 是一种 Rabbit 抗体,靶向 DUSP1。Anti-DUSP1 Antibody (4S873) 可用于 WB,IHC,IP,FCM,ICC/IF。
Anti-DUSP1 Antibody (4S873) 是一种 Rabbit 抗体,靶向 DUSP1。Anti-DUSP1 Antibody (4S873) 可用于 WB,IHC,IP,FCM,ICC/IF。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,480 | 5日内发货 | |
100 μL | ¥ 2,495 | 5日内发货 |
产品描述 | Anti-DUSP1 Antibody (4S873) is a Rabbit antibody targeting DUSP1. Anti-DUSP1 Antibody (4S873) can be used in WB,IHC,IP,FCM,ICC/IF. |
别名 | VH1, VH 1, Serine/threonine specific protein phosphatase, Ptpn16, PTPN10, PTPN 10, Protein tyrosine phosphatase CL100, Mkp1, MKP 1, Mitogen activated protein kinase phosphatase 1, MAP kinase phosphatase 1, HVH1, HVH 1, EC 3.1.3.48, EC 3.1.3.16, DUSP-1, DUSP1, Dual specificity protein phosphatase hVH1, Dual specificity protein phosphatase 1, Dual Specificity Phosphatase 1, CL100, CL 100, 3ch134 |
Ig Type | IgG |
克隆号 | 4S873 |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of DUSP1 on mouse spleen lysates using anti-DUSP1 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DUSP1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-DUSP1 antibody. Counter stained with hematoxylin. 4. Flow cytometric analysis of HepG2 cells with DUSP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000<:br>IHC: 1:50-200<:br>FCM: 1:50-100<:br>ICC/IF: 1:50-200 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | A key element in the pathway involved in the transduction of signals from activated protein-tyrosine kinase transmembrane receptors has been identified as the family of mitogen-activated protein kinases (MAP kinases). The most well known of these Ser/Thr kinases are ERK 1 and ERK 2. Mitogenic stimulation of cells triggers the activation of MAP kinases through phosphorylation of both tyrosyl (Y185) and threonyl (T183) residues. Phosphorylation of the T183 and Y185 ERK regulatory site is mediated by MAP kinase (MEK), which in turn is regulated by the proto-oncogene product Raf. Two highly related phosphatases, designated MKP-1 and MKP-2, exhibit 59% sequence identity at the amino acid level and oppose the action of MEK by downregulating the kinase activity of ERK 1 and ERK 2. MAP kinase phosphatase-1 and -2 proteins function by dephosphorylating ERK 1 and ERK 2 at their T-E-Y regulatory motif. An additional phosphatase encoded by the DUSP2 gene, designated PAC-1, also functions to downregulate ERK 1 and ERK 2 kinase activity. PAC-1 is a nuclear protein whose expression is strongly induced in response to mitogen. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 40 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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