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Anti-Drosha Antibody (5J107)
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产品编号 TMAC-01185
别名 RNC_HUMAN, RNASEN, RNaseIII, RNASE3L, RNase3, RNase III, RNase 3, RN3, RN 3, RibonucleaseIII, Ribonuclease type III nuclear, Ribonuclease III nuclear, Ribonuclease III, Ribonuclease 3, RANSE3L, Putative ribonuclease III, Putative protein p241 which interacts with transcription factor Sp1, Protein Drosha, p241, Nuclear RNase III Drosha, HSA242976, Etohi2, Drosha ribonuclease type III, Drosha double stranded RNA specific endoribonuclease, DROSHA
Anti-Drosha Antibody (5J107) 是一种 Rabbit 抗体，靶向 Drosha。Anti-Drosha Antibody (5J107) 可用于 WB,ICC,IF,IHC,FCM。
Anti-Drosha Antibody (5J107)
Anti-Drosha Antibody (5J107)
还可以产品编号 TMAC-01185 别名 RNC_HUMAN, RNASEN, RNaseIII, RNASE3L, RNase3, RNase III, RNase 3, RN3, RN 3, RibonucleaseIII, Ribonuclease type III nuclear, Ribonuclease III nuclear, Ribonuclease III, Ribonuclease 3, RANSE3L, Putative ribonuclease III, Putative protein p241 which interacts with transcription factor Sp1, Protein Drosha, p241, Nuclear RNase III Drosha, HSA242976, Etohi2, Drosha ribonuclease type III, Drosha double stranded RNA specific endoribonuclease, DROSHA
Anti-Drosha Antibody (5J107) 是一种 Rabbit 抗体，靶向 Drosha。Anti-Drosha Antibody (5J107) 可用于 WB,ICC,IF,IHC,FCM。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,490 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-Drosha Antibody (5J107) is a Rabbit antibody targeting Drosha. Anti-Drosha Antibody (5J107) can be used in WB,ICC,IF,IHC,FCM. |
| 别名 | RNC_HUMAN, RNASEN, RNaseIII, RNASE3L, RNase3, RNase III, RNase 3, RN3, RN 3, RibonucleaseIII, Ribonuclease type III nuclear, Ribonuclease III nuclear, Ribonuclease III, Ribonuclease 3, RANSE3L, Putative ribonuclease III, Putative protein p241 which interacts with transcription factor Sp1, Protein Drosha, p241, Nuclear RNase III Drosha, HSA242976, Etohi2, Drosha ribonuclease type III, Drosha double stranded RNA specific endoribonuclease, DROSHA |
| Ig Type | IgG |
| 交叉反应 | Human |
| 验证活性 | 1. Western blot analysis of Drosha on Hela cell and SiHa cell lysates using anti-Drosha antibody at 1/500 dilution. 2. Western blot analysis of Drosha on K562 cell lysates using anti-Drosha antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Drosha antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Drosha antibody. Counter stained with hematoxylin. 5. ICC staining Drosha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Drosha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Drosha in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Jurkat cells with Drosha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
| 应用 | |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The ribonuclease III superfamily represents a structurally distinct group of double-strand-specific endonucleases with essential roles in RNA maturation, RNA decay, and gene silencing. Initial cleavage of microRNAs is catalysed by Drosha, a nuclease of the RNase III family, which acts on primary miRNA transcripts (pri-miRNAs) in the nucleus. Human Drosha is a component of two multi-protein complexes. The larger complex contains multiple classes of RNA-associated proteins including RNA helicases, proteins that bind double-stranded RNA, novel heterogeneous nuclear ribonucleoproteins and the Ewing's sarcoma family of proteins. The smaller complex is composed of Drosha and the double-stranded-RNA-binding protein, DGCR8. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 159 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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