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Anti-DPP4/CD26 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 DPP4/CD26。Anti-DPP4/CD26 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF,FCM。
Anti-DPP4/CD26 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 DPP4/CD26。Anti-DPP4/CD26 Polyclonal Antibody 可用于 IHC-P,IHC-Fr,IF,FCM。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,175 | 5日内发货 | |
100 μL | ¥ 1,970 | 5日内发货 | |
200 μL | ¥ 2,795 | 5日内发货 |
产品描述 | Anti-DPP4/CD26 Polyclonal Antibody is a Rabbit antibody targeting DPP4/CD26. Anti-DPP4/CD26 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
交叉反应 | Human,Rat (predicted:Mouse,Pig,Cow,Horse,Rabbit) |
验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD26) Polyclonal Antibody, Unconjugated (TMAB-00567) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Blank control (blue): Raji (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD26 antibody (TMAB-00567), Dilution: 3 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. 3. Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CD26) Polyclonal Antibody, Unconjugated (TMAB-00567) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
应用 | |
推荐剂量 | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 3μg/Test |
抗体种类 | Polyclonal |
宿主来源 | Rabbit |
亚细胞定位 | Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid. |
构建方式 | Hybridoma Polyclonal Antibody |
纯化方式 | Protein A purified |
性状 | Liquid |
缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
研究背景 | The DPP4 gene encodes dipeptidyl peptidase 4, which is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic type II transmembrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Dipeptidyl peptidase 4 is highly involved in glucose and insulin metabolism, as well as in immune regulation. This protein was shown to be a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV), and protein modeling suggests that it may play a similar role with SARS-CoV-2, the virus responsible for COVID-19. [provided by RefSeq, Apr 2020] |
免疫原 | KLH conjugated synthetic peptide: human CD26 |
抗原种属 | Human |
基因ID | |
Uniprot ID | |
研究领域 | Response to hypoxia,DPPs,CD,CD,Adhesion,Non-lineage,HIV-related,Hypoxia,SARS Coronavirus,B Lymphocytic Lineage,T Lymphocytic Lineage |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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