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Anti-Dnmt3a Antibody (1X206)

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Anti-Dnmt3a Antibody (1X206)
产品编号 TMAC-01169
别名 TBRS, OTTHUMP00000201149, MCMT, M.HsaIIIA, DNMT3A2, Dnmt3a, DNMT 3a, DNMT, DNM3A_HUMAN, DNA MTase HsaIIIA, DNA methyltransferase HsaIIIA, DNA methyltransferase 3a, DNA methyltransferase 3 alpha, DNA cytosine methyltransferase 3A2, DNA (cytosine-5)-methyltransferase 3A, DNA (cytosine 5) methyltransferase 3A, DNA (cytosine 5) methyltransferase 3 alpha

Anti-Dnmt3a Antibody (1X206) 是一种 Rabbit 抗体，靶向 Dnmt3a。Anti-Dnmt3a Antibody (1X206) 可用于 WB,ICC/IF,IHC,FCM。

Anti-Dnmt3a Antibody (1X206)

Anti-Dnmt3a Antibody (1X206)

Rating icon 还可以
Anti-Dnmt3a Antibody (1X206)
产品编号 TMAC-01169 别名 TBRS, OTTHUMP00000201149, MCMT, M.HsaIIIA, DNMT3A2, Dnmt3a, DNMT 3a, DNMT, DNM3A_HUMAN, DNA MTase HsaIIIA, DNA methyltransferase HsaIIIA, DNA methyltransferase 3a, DNA methyltransferase 3 alpha, DNA cytosine methyltransferase 3A2, DNA (cytosine-5)-methyltransferase 3A, DNA (cytosine 5) methyltransferase 3A, DNA (cytosine 5) methyltransferase 3 alpha

Anti-Dnmt3a Antibody (1X206) 是一种 Rabbit 抗体，靶向 Dnmt3a。Anti-Dnmt3a Antibody (1X206) 可用于 WB,ICC/IF,IHC,FCM。

规格价格库存数量
50 μL
¥ 1,490
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100 μL
¥ 2,480
5日内发货
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产品介绍

生物活性
产品描述
Anti-Dnmt3a Antibody (1X206) is a Rabbit antibody targeting Dnmt3a. Anti-Dnmt3a Antibody (1X206) can be used in WB,ICC/IF,IHC,FCM.
别名TBRS, OTTHUMP00000201149, MCMT, M.HsaIIIA, DNMT3A2, Dnmt3a, DNMT 3a, DNMT, DNM3A_HUMAN, DNA MTase HsaIIIA, DNA methyltransferase HsaIIIA, DNA methyltransferase 3a, DNA methyltransferase 3 alpha, DNA cytosine methyltransferase 3A2, DNA (cytosine-5)-methyltransferase 3A, DNA (cytosine 5) methyltransferase 3A, DNA (cytosine 5) methyltransferase 3 alpha
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of Dnmt3a on different lysates using anti-Dnmt3a antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: Human brain, Lane 3: Human heart.
2. ICC staining Dnmt3a in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Dnmt3a in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. ICC staining Dnmt3a in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
应用
WB
ICC/IF
IHC
FCM
推荐剂量
WB: 1:1000-5000; ICC/IF: 1:50-200
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q9Y6K1
化学信息
分子量Theoretical: 120 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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