Anti-DARS Antibody (5Z51)

Name: Anti-DARS Antibody (5Z51)
Brand: TargetMol
SKU: TMAY-02711
Rating: 4.5 (1 reviews)

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产品编号 TMAY-02711

Anti-DARS Antibody (5Z51) 是一种 Mouse 抗体，靶向 DARS。Anti-DARS Antibody (5Z51) 可用于 ELISA,FCM,ICC/IF。

Anti-DARS Antibody (5Z51)

还可以

产品编号 TMAY-02711

Anti-DARS Antibody (5Z51) 是一种 Mouse 抗体，靶向 DARS。Anti-DARS Antibody (5Z51) 可用于 ELISA,FCM,ICC/IF。

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生物活性

产品描述	Anti-DARS Antibody (5Z51) is a Mouse antibody targeting DARS. Anti-DARS Antibody (5Z51) can be used in ELISA,FCM,ICC/IF.
Ig Type	Monoclonal Mouse IgG1
克隆号	5Z51
交叉反应	Human
验证活性	1. Flow cytometric analysis of Human DARS expression on HepG2 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human DARS, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of Human DARS in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human DARS monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cytoplasm.
应用	ELISA,FCM,ICC/IF
推荐剂量	ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100
抗体种类	Monoclonal
宿主来源	Mouse
构建方式	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human DARS (rh DARS; TMPY-03393; P14868; Met1-Pro501).
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS
研究背景	Aspartate tRNA ligase 1, also known as DARS, is part of a multienzyme complex of aminoacyl-tRNA synthetases. It belongs to the class-II aminoacyl-tRNA synthetase family. DARS charges its cognate tRNA with aspartate during protein biosynthesis. DARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid(AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	Recombinant Human DARS protein (TMPY-03393)
抗原种属	Human

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.