Anti-DARS Antibody (5Z51)

Name: Anti-DARS Antibody (5Z51)
Brand: TargetMol
SKU: TMAY-02711
Rating: 4.5 (1 reviews)

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产品编号 TMAY-02711

别名 HBSL, aspRS, aspartyl-tRNA synthetase

Anti-DARS Antibody (5Z51) 是一种 Mouse 抗体，靶向 DARS。Anti-DARS Antibody (5Z51) 可用于 ELISA,FCM,ICC/IF。

Anti-DARS Antibody (5Z51)

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Anti-DARS Antibody (5Z51)

还可以

产品编号 TMAY-02711 别名 HBSL, aspRS, aspartyl-tRNA synthetase

Anti-DARS Antibody (5Z51) 是一种 Mouse 抗体，靶向 DARS。Anti-DARS Antibody (5Z51) 可用于 ELISA,FCM,ICC/IF。

规格	价格	库存	数量
100 μL	¥ 1,694	5日内发货

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生物活性

产品描述	Anti-DARS Antibody (5Z51) is a Mouse antibody targeting DARS. Anti-DARS Antibody (5Z51) can be used in ELISA,FCM,ICC/IF.
别名	HBSL, aspRS, aspartyl-tRNA synthetase
Ig Type	Mouse IgG1
克隆号	5Z51
交叉反应	Human
验证活性	1. Flow cytometric analysis of Human DARS expression on HepG2 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human DARS, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of Human DARS in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human DARS monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cytoplasm.
应用	ELISA,FCM,ICC/IF
推荐剂量	ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100
抗体种类	Monoclonal
宿主来源	Mouse
构建方式	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human DARS (rh DARS; TMPY-03393; P14868; Met1-Pro501).
纯化方式	Protein A
性状	Liquid
缓冲液	0.2 μm filtered solution in PBS
研究背景	Aspartate tRNA ligase 1, also known as DARS, is part of a multienzyme complex of aminoacyl-tRNA synthetases. It belongs to the class-II aminoacyl-tRNA synthetase family. DARS charges its cognate tRNA with aspartate during protein biosynthesis. DARS catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid(AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

偶联与修饰

偶联	Unconjugated

抗原信息

免疫原	Recombinant Protein: Human DARS protein (TMPY-03393)
抗原种属	Human

存储&运输

储存方式	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
运输方式	Shipping with blue ice.